Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Antiulcerogenic Activity of Li-Zhong Decoction on Duodenal Ulcers Induced by Indomethacin in Rats: Involvement of TLR-2/MyD88 Signaling Pathway

doi: 10.1155/2020/6538156

Figure Lengend Snippet: List of primers used in qRT-PCR.

Article Snippet: The sections were incubated with rabbit anti-TLR2 antibody (1 : 100 dilution; Bioss antibodies, Beijing, China) or rabbit anti-MyD88 antibody (1 : 100 dilution; Bioss antibodies, Beijing, China) overnight at 4°C and then with HRP-conjugated goat anti-rabbit secondary antibody (1 : 100 dilution; Bioss antibodies, Beijing, China).

Techniques: Sequencing

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Antiulcerogenic Activity of Li-Zhong Decoction on Duodenal Ulcers Induced by Indomethacin in Rats: Involvement of TLR-2/MyD88 Signaling Pathway

doi: 10.1155/2020/6538156

Figure Lengend Snippet: Effects of LZD on levels of TLR-2 (a) and MyD88 (b) mRNA expression during prevention of IND‐induced duodenal ulceration. Levels of TLR-2 and MyD88 mRNA were determined by qRT-PCR assay. β -Actin mRNA was used as internal control for equal loading. Results are expressed as mean ± SEM, n = 3. Asterisks and pound signs indicate significant differences: ∗∗ P < 0.01 versus normal control (NC); ## P < 0.01 versus negative control (IND).

Article Snippet: The sections were incubated with rabbit anti-TLR2 antibody (1 : 100 dilution; Bioss antibodies, Beijing, China) or rabbit anti-MyD88 antibody (1 : 100 dilution; Bioss antibodies, Beijing, China) overnight at 4°C and then with HRP-conjugated goat anti-rabbit secondary antibody (1 : 100 dilution; Bioss antibodies, Beijing, China).

Techniques: Expressing, Quantitative RT-PCR, Negative Control

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Antiulcerogenic Activity of Li-Zhong Decoction on Duodenal Ulcers Induced by Indomethacin in Rats: Involvement of TLR-2/MyD88 Signaling Pathway

doi: 10.1155/2020/6538156

Figure Lengend Snippet: Effects of LZD on levels of TLR-2 and MyD88 protein expression during prevention of IND induced duodenal ulceration. (a) Representative immunoblots for TLR-2 and MyD88 proteins as measured by western blot analysis using specific antibodies. Loading control was monitored by β -actin immunoblotting. Quantitative analysis derived from densitometric scans of western blots of TLR-2 (b) and MyD88 (c) proteins of duodenal mucosa. Results are expressed as mean ± SEM, n = 3. Asterisks and pound signs indicate significant differences: ∗∗ P < 0.01 versus normal control (NC); ## P < 0.01 versus negative control (IND).

Article Snippet: The sections were incubated with rabbit anti-TLR2 antibody (1 : 100 dilution; Bioss antibodies, Beijing, China) or rabbit anti-MyD88 antibody (1 : 100 dilution; Bioss antibodies, Beijing, China) overnight at 4°C and then with HRP-conjugated goat anti-rabbit secondary antibody (1 : 100 dilution; Bioss antibodies, Beijing, China).

Techniques: Expressing, Western Blot, Derivative Assay, Negative Control

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Antiulcerogenic Activity of Li-Zhong Decoction on Duodenal Ulcers Induced by Indomethacin in Rats: Involvement of TLR-2/MyD88 Signaling Pathway

doi: 10.1155/2020/6538156

Figure Lengend Snippet: Localization of TLR-2 and MyD88 in duodenal tissues after IND administration and effect of LZD thereon. (a) Representative pictures showing the immunohistochemical analysis of TLR-2 and MyD88 in sections of duodenal mucosa obtained from rats in normal control, negative control, ESO, and LZD groups. The black arrows denote the immunopositive expression parts of TLR-2 and MyD88. Magnification 200x. (b, c) Quantitative analysis of the immunohistochemical signals as measured by IOD values. Results are expressed as the mean ± SEM ( n = 3). Asterisks and pound signs indicate significant differences: ∗∗ P < 0.01 versus normal control (NC); ## P < 0.01 versus negative control (IND).

Article Snippet: The sections were incubated with rabbit anti-TLR2 antibody (1 : 100 dilution; Bioss antibodies, Beijing, China) or rabbit anti-MyD88 antibody (1 : 100 dilution; Bioss antibodies, Beijing, China) overnight at 4°C and then with HRP-conjugated goat anti-rabbit secondary antibody (1 : 100 dilution; Bioss antibodies, Beijing, China).

Techniques: Immunohistochemical staining, Negative Control, Expressing

Journal: Molecular medicine reports

Article Title: Possible role of miR-204 in optic nerve injury through the regulation of GAP-43.

doi: 10.3892/mmr.2017.8341

Figure Lengend Snippet: Figure 1. Expression levels of miR‑204, GAP‑43, TLR4, MyD88 and NF‑κB. Relative levels of miR‑204, GAP‑43, TLR4, MyD88 and NF‑κB in the retina of each group demonstrated by (A) reverse transcription‑quantitative polymerase chain reaction and (B) western blot analyses. (C) Quantification of protein levels. Compared with the normal SD rats, the level of miR‑204 in SD rats with optic nerve injury was significantly increased (**P<0.05, ***P<0.01) and the mRNA level of GAP‑43 was significantly inhibited by optic nerve injury (**P<0.05, ***P<0.01). miR‑204 increased the gene levels of TLR4/MyD88/NF‑κB, and decreased the mRNA and protein expression of GAP‑43. miR, microRNA; GAP‑43, growth‑associated protein-43; TLR4, toll‑like receptor 4; MyD88, myeloid differentiation factor 88; NF‑κB, nuclear factor‑κB; SD, Sprague‑Dawley.

Article Snippet: Primary antibodies against GAP-43, TLR4, MyD88 and NF-κB were from Abcam (Cambridge, UK); the ChemiDocTM XRS gel imaging system and fluorescence qPCR instrument were from Bio-Rad Laboratories, Inc. (Hercules, CA, USA); SYBR-Green qPCR SuperMix was from Invitrogen; Thermo Fisher Scientific, Inc. (Waltham, MA, USA); the TUNEL apoptosis kit was from Beyotime Institute of Biotechnology (Haimen, China); the mini double vertical electrophoresis and mini transfer electrophoresis apparatus were from Beijing 61 Instrument Factory (Beijing, China); the inverted fluorescence microscope was from Leica Microsystems, Inc. (Buffalo Grove, IL, USA).

Techniques: Expressing, Polymerase Chain Reaction, Western Blot